Sharp DM-4500 Instruções de Operação descarregar pdf (Página 75)

11. Troubleshooting

Problem

Bright field

The specimen can not be brought into focus.

Dark field

No definite DF contrast is possible.

The image is unevenly or not uniformly

illuminated.

Undesirable stray light.

Phase contrast

No phase contrast is possible.

Cause/remedy

Use the correct immersion medium.

Lay the specimen with the cover glass toward

the top.

Make sure that the cover glass thickness is

correct and that it meets the specifications on

the objective.

Be sure that a DF objective is being used.

The objective aperture setting is too high

(maximum 0.75). If necessary, reduce the

objective aperture using the iris diaphragm on

the objective.

Check the condenser centering.

The magnification is too weak.

Use a higher magnification.

Clean the specimen and neighboring lens sur-

faces (→

p. 77).

The specimen is too thick.

The cover glass is not placed planar.

Check the centering of the light rings

(→ p. 44).

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Sharp DM-4500 Instruções de Operação Página 75